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DOI: https://doi.org/10.21897/rta.v18i2.715

Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.)

Standardization of protocol to evaluate peroxidase activity in tree tomato (solanum betaceum cav.)

Margareth Santander, Diego Mejía, Oswaldo Osorio

Resumen


El objetivo de esta investigación fue obtener un protocolo de análisis de la actividad enzimática de Peroxidasa (POD) en pulpa de tomate de árbol (Solanum betaceum Cav.). Se evaluaron variables en el proceso de extracción como: el porcentaje de pulpa (PP), de 20 a 50% y el tiempo de centrifugación (TC) de 10 a 30 minutos; mientras que en la medición se varió el volumen de extracto (VE), de 30 a 90 L. Se utilizó un diseño experimental de composición central 23 más puntos estrellas y puntos centrales con metodología de superficie de respuesta con dos repeticiones y un total de 32 unidades experimentales. Para la optimización, la mayor actividad enzimática se presentó empleando un PP de 60%, un TC de 34 min y un VE de 110 µl.

Palabras clave


Enzimas; actividad peroxidasa; frutales; pulpa; protocolo

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Referencias


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